Transfection Transfection is the use of chemical liposomes, chemicals or mechanical electroporation means to transfer nucleic acids into cells in order to alter mRNA Molecular biology research articles or the genetic makeup of a genome i.
Most enzymes are proteins, although a few are catalytic RNA molecules. The laboratory work that goes into the study of blood is frequently performed by a medical technologist. Compound made inside human body stops viruses from replicating 20 June — A team of researchers has identified the mode of action of viperin, a naturally occurring enzyme in humans and other mammals that is known to have antiviral effects on viruses such as West Nile, hepatitis C, rabies, and HIV.
Other kinds of medical research include pre-clinical research, for example on animalsand basic medical research, for example in genetics.
Reverse genetics Reverse genetics determines the phenotype that results from a specifically engineered gene. Located upstream of the multiple cloning site are the promoter regions and the transcription start site which regulate the expression of cloned gene.
Related Journals of Evolutionary Biology Journal of Evolutionary BiologyBMC Evolutionary Biology, Evolutionary Biology, Trends in Evolutionary Biology Epigenetics In geneticsepigenetics is the study of cellular and physiological trait variations that are not caused by changes in the DNA sequence; in layman's terms, epigenetics is essentially the study of external or environmental factors that turn genes on and off and affect how cells read genes.
Gross anatomy also includes the branch of superficial anatomy. All experiments and products are controlled by the U. Hence, epigenetic research seeks to describe dynamic alterations in the transcriptional potential of a cell.
A similar process to transduction of Molecular biology research articles plasmid DNA into bacteria or non-animal eukaryotic cells is called transformation. Liposomes are small capsules made of polymers similar to those that make up the cellular membrane. This ultimately leads to lower manufacturing costs of the transfection reagent since lower concentrations can be used per test sample.
Proteins blotted on to the PVDF or nitrocellulose membrane are probed for modifications using specific substrates. Using western blotting techniques allows not only detection but also quantitative analysis.
A successful transfection reagent also readily loads with a high ratio of active pharmaceutical ingredient API to delivery agent ratio. Human anatomy is one of the basic essential sciences of medicine.
Related Journals of Mycology Developmental BiologyCurrent Topics in Developmental BiologyMedical Mycology, Studies in Mycology, Journal of Medical and Veterinary Mycology, Japanese Journal of Medical Mycology, Current topics in medical mycology Population Biology Population biology is a study of populations of organisms, especially the regulation of population size, life history traits such as clutch size, and extinction.
There is also a long tradition of studying biomolecules "from the ground up" in biophysics.
It is a multi-disciplinary science with many subspecialties including clinical pharmacology, cardiovascular pharmacologybehavioural pharmacology, neuropsychopharmacology, pharmacogeneticsand pharmacoeconomics.
In this process RNA is separated based on size and is then transferred to a membrane that is then probed with a labeled complement of a sequence of interest. Introducing DNA into bacterial cells can be done by transformation via uptake of naked DNA, conjugation via cell-cell contact or by transduction via viral vector.
Patricia Thomas, developer of the RNA blot which then became known as the northern blot, actually didn't use the term. New neuron-like cells allow investigation into synthesis of vital cellular components 22 January — Using a new method to create synthetic neurons, a team of researchers from Penn State explores how the human brain makes a metabolic building block essential for the survival of all living organisms.
The PCR technique can be used to introduce restriction enzyme sites to ends of DNA molecules, or to mutate particular bases of DNA, the latter is a method referred to as site-directed mutagenesis. Related Journals of Ecology Trends in Ecology and EvolutionEcology Letters, Molecular Ecology, Annual Review of Ecology, Evolution, and Systematics Bioethics Bioethics is the study of the typically controversial ethical issues emerging from new situations and possibilities brought about by advances in biology and medicine.
Gene therapy can be used to replace a mutated gene with the correct copy of the gene, to inactivate or knockout the expression of a malfunctioning gene, or to introduce a foreign gene to the body to help fight disease.
The project was started by the U. The term population biology is often used interchangeably with population ecologyalthough 'population biology' is more frequently used when studying diseases, virusesand microbes, and 'population ecology' is used more frequently when studying plants and animals.
New insights into how cellulose is built could indicate how to break it apart for biofuels 26 March — A comprehensive look at how plants build cellulose, the primary building block of the walls of most plant cells that is used in a wide variety of manmade materials, could have important implications for its use in biofuels.
The study of the chemistry behind biological processes and the synthesis of biologically active molecules are examples of biochemistry. The relationship between scales is not simple or necessarily direct, and sometimes become combinatorial, so that systems approaches are essential to evaluate these relationships quantitatively and qualitatively.
A biologist specializing in mycology is called a mycologist. Eastern blot The eastern blotting technique is used to detect post-translational modification of proteins.Biological Research, formerly Archives of Experimental Medicine and Biology, was founded in and transferred to BioMed Central in An electronic archive of articles published between and can be found in the SciELO database.
Molecular biology / m ə ˈ l ɛ k j ʊ l ər / is a branch of biology that concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.
Writing in Nature inWilliam Astbury described molecular biology as. BMC Molecular Biology is an open access journal publishing original peer-reviewed research articles in all aspects of DNA and RNA in a cellular context, encompassing investigations of chromatin, replication, recombination, mutation, repair, transcription, translation and RNA processing and function.
The most downloaded articles from Journal of Molecular Biology in the last 90 days. The most downloaded articles from Journal of Molecular Biology in the last 90 days.
Menu. Search. Search. Search in: All. Webpages. it appears on ScienceDirect linked to the original research article in this journal. A new virtual issue on Omics of Lipids, Glycans and Polar Metabolites is a collaboration between the Journal of Biological Chemistry, Molecular and Cellular Proteomics and the Journal of Lipid Research.
Transfection. Transfection is the use of chemical (liposomes, chemicals) or mechanical (electroporation) means to transfer nucleic acids into cells in order to alter mRNA expression or the genetic makeup of a genome (i.e. stable expression of exogenous genes or gene knockout).Download